HPV Serology Operating Procedures
The HPV Serology Laboratory leads an HPV Serology Standardization initiative in collaboration with the National Cancer Institute and the Bill and Melinda Gates Foundation. We invite you to download and use the following standard operating procedures in your own serology laboratory.
Protection against Human Papillomavirus (HPV) infection after vaccination is believed to be mediated by HPV-specific antibodies. Antibody responses in HPV prophylactic vaccine trials have been assessed using different methods.
The lack of standardized assays, procedures, and reagents accessible to the scientific community has precluded the comparison of different studies evaluating immunogenicity of HPV vaccines. The HPV Serology Laboratory was established to address some of these needs and advance HPV assay standardization.
General Laboratory Procedures
- 20000 Bacterial Plasmid Culture1.06 MB 2/14/2023View
- 293TT Cell Culturing and Maintenance1.74 MB 1/7/2025View
- Bacterial Plasmid Culture1.13 MB 1/7/2025View
- Bacterial Transformation1.22 MB 1/7/2025View
- Plasmid DNA Transfection in HEK293TT for VLP Production and Purification4.34 MB 1/7/2025View
- Plasmid Purification Using a Zymo Research Kit1.74 MB 1/7/2025View
- Preparation of Bacterial Glycerol Stock for Plasmid Purification2.12 MB 1/7/2025View
- Procedure for Separating Serum and Plasma from Whole Blood547.18 KB 1/7/2025View
- Reagent Preparation1.3 MB 12/3/2024View
Equipment
- 26000 Biosafety Cabinet Use and Maintenance1.19 MB 6/9/2022View
- 26001 Operation, Use, and Maintenance of C02 Incubators966.23 KB 6/9/2022View
- 26002 Use and Maintenance of a BioTek Plate Washer1.85 MB 6/9/2022View
- 26003 Use and Maintenance of a Molecular Devices M5 Plate Reade1.03 MB 6/9/2022View
- 26004 Use and Maintenance of the Cellometer Cell Counter1.01 MB 6/9/2022View
- 26007 Use and Maintenance of the Fisher Scientific Isotemp GDP10 Water Bath693.49 KB 6/9/2022View
- 26008 Use and Maintenance of Incubator Shakers2.3 MB 6/9/2022View
- 26009 Use and Maintenance of Pipettes1.13 MB 6/9/2022View
- 26010 Use and Maintenance of the Thermomixer962.11 KB 6/9/2022View
- 26011 Use and Maintenance of a pH Meter1.38 MB 6/9/2022View
- 26012 Use and Maintenance of an Analytical & Precision Balance1.27 MB 6/9/2022View
- 26013 Use and Maintenance of -20C Freezers in the HPV Serology Laboratory1.04 MB 6/9/2022View
- 26014 Use and Maintenance of Convection Incubator1021.95 KB 6/9/2022View
- 26015 Use and Maintenance of the Inverted Microscope1003.22 KB 6/9/2022View
- 26016 Use and Maintenance of the Water Purification Systems1.36 MB 6/9/2022View
- 26022 Use and Maintenance of the Combination Refrigerator and Freezer972.37 KB 6/9/2022View
- 26023 Use and Maintenance of the Agilent Bioanalyzer1.86 MB 6/9/2022View
- 26027 Use and Maintenance of the FYRITE Gas Analyzer687.83 KB 6/9/2022View
- 26028 General Use and Maintenance of the MESO QuickPlex SQ 120594.55 KB 6/9/2022View
- 26030 Use and Maintenance of -80C Freezers1.02 MB 6/9/2022View
- 26035 Verification and Calibration of Laboratory Timers472.8 KB 6/9/2022View
Laboratory Assays
- 20001 HEK293TT Cell Culturing and Maintenance1.82 MB 6/9/2022View
- 20002 Preparation of Bacterial Glycerol Stock for Plasmid Purification2.4 MB 6/9/2022View
- 20003 Procedure for Separating Serum and Plasma from Whole Blood602.13 KB 6/9/2022View
- 20006 Ripcord pDNA Transfection3.07 MB 6/9/2022View
- 30009 BCA Protein Assay1.46 MB 6/9/2022View
- ELISA to Determine VLP Specificity1.78 MB 12/3/2024View
- HPV Antibody ELISA2.69 MB 12/3/2024View
- HPV Neutralization Assay for Titer Determination5.2 MB 12/3/2024View
- Protein Analysis of Virus-Like Particles (VLPs) using the Agilent 2100 Bioanalyzer966.73 KB 12/3/2024View
- SARS CoV-2 Avidity ELISA1.77 MB 12/3/2024View
- SARS-CoV-2 Antibody ELISA2.09 MB 12/3/2024View