Given an input sequence or list of sequences as well as a CRISPR system with a defined spacer length and PAM sequence, sgRNA Scorer will identify putative sites and assign a predicted activity based on our new support vector machine model. In addition, an option exists to select sites with minimal off-target activity based on the CasFinder algorithm as well, enabling target sites to be selected with high predicted specificity and activity.
In this 2.0 version, we combined the support vector machine models for Cas9 S. pyogenes and Cas9 S. thermophilus 1 into a single pan-ortholog model and have demonstrated experimentally that it works with varying degree of success for Cas9s from S. aureus and S. thermophilus 3 as well as the newly characterized CRISPR system Cpf1.
