Quantitative Assessment of Lymphoid Tissue Fibrosis

  • Performs immunohistochemistry to determine the quantity of  extracellular matrix deposits accumulated within secondary lymphoid tissues. 
  • Generates high-resolution digital images of each immunohistochemistry stained sample. 
  • Provides relevant data electronically, including a report with interpretations and conclusions. 

The process: 

  • Collagen I, Collagen III, or Fibronectin immunohistochemistry is performed to ascertain the extent of extracellular matrix deposition (i.e., fibrosis) accumulated within secondary lymphoid tissues. 
    • To ensure consistency among assay batches, we utilize an automated immunohistochemistry system. 
  • High-resolution digital images of each IHC stained sample are generated using the Aperio ScanScope CS System. 
  • Regions of interest of a defined size are highlighted on each digital image using the Aperio ImageScope software regions tool. 
  • High-resolution images of regions of interest are generated using a custom software program created by the Imaging and Visualization Group that automatically extracts regions of interest from the digital slide annotation layer. 
  • Under the guidance of a subject matter expert, the core provides comprehensive quantitative image analysis using a custom series of unique actions in Photoshop with Fovea tools to determine the percent area in each high-resolution image that is occupied by fibrotic collagen. 

Includes sufficient basic consultation with a subject matter expert. 

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A portrait photo
Claire Deleage Head, Tissue Analysis and Retroviral Protein Chemistry Core