In Person Event
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vEM101 workshop graphic

Volume electron microscopy (vEM) is quietly revolutionizing cell biological imaging by enabling the nm-scale 3D visualization of cells and tissues, and catalyzing discoveries in various experimental systems. Scientists in the vEM community have made innovations at different steps in the vEM experimental pipeline, creating a wide variety of protocols, many useful and bespoke but few that are standard and widely used.

In an effort to demystify vEM to newcomers to the field, this 2-day workshop will offer an up-close and in-person view of sample preparation, imaging, and image processing in a leading vEM laboratory. Held in conjunction with BioEM webinar talks as well as vendor presentations, this will be an opportunity to come away with an understanding of the basics of this exciting field as well as a set of standard protocols to execute a basic vEM experiment.

If you are new to or considering volume EM, a young researcher using vEM, or an EM enthusiast curious about vEM, this will an opportunity both to understand experimental aspects of the science as well as to network with fellow vEM community members.

Application

You must apply to this workshop as seats are limited. The workshop is open to U.S. participants only. It is free to attend and expenses for participants' stay will be defrayed. 

NOTE (1): Due to practical constraints, we will not be able to schedule personalized 1:1 “full hands-on” sessions. Rather, this demo experience will serve as a thorough introduction and help you decide which applications to pursue in greater depth.

NOTE (2): Some of the demos may be remote.

Contact

Contact
Job Position

Experience

Years in EM
How long have you been in EM or vEM?

Check if you've done EM only, no vEM.

Which EM tools do you have experience with?

Tools experience
SEM
TEM
ET
FIB-SEM
SBF-SEM
AT
None

Why do you want to attend this workshop? (150 words max)

Interest in vEM pipeline

Volume EM pipelines include many steps, and each of those steps can be executed in multiple ways, using various tools. To get a better sense of what excites YOU the most, please answer the following questions. We will try our best to tailor the program to create maximum overlap between your needs and our resources.

Check the areas you're interested in

All areas
Sample Prep
Imaging
Image Processing

Sort by interest

Drag and drop each element within each table to arrange in order of most interest to least. Each table is focused on one section of the vEM pipeline.

Note: Some options will be demo'd virtually.

Sample prep Weight
High Pressure Freezing/Freeze Substitution
Robotic room temp sample prep
ATUMtome
ARTOS3D microtome
Imaging Weight
Electron Tomography
FIB-SEM
SBF-SEM
Array Tomography
CryoFIB-SEM
Image processing Weight
Open Source Tools
Amira
Arivis
Other (please specify in Notes/Thoughts section)

Disclaimer: Photographs, video, and live streaming may occur during this conference for social media and other event publicity purposes. Your registration and/or participation in the meeting indicates your consent to be photographed and grants the National Institutes of Health the right to copy, distribute, and/or exhibit name(s) and/or picture(s) of you.

OMB No.: 0925-0740
Expiration Date:  09/30/2025

Public reporting burden for this collection of information is estimated to average 5 minutes per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. An agency may not conduct or sponsor, and a person is not required to respond to, a collection of information unless it displays a currently valid OMB control number. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to: NIH, Project Clearance Branch, 6705 Rockledge Drive, MSC 7974, Bethesda, MD 20892-7974, ATTN: PRA (0925-0740). Do not return the completed form to this address.