The Antibody Characterization Laboratory is a reference program that develops antibodies for the scientific community, including extensive antibody characterization data and protocols.
The laboratory uses an array of techniques to determine the properties of antibodies and other renewable reagents that can be used in cancer-related research. All the reagents, associated data, and standard operating procedures are readily available as part of the National Cancer Institute’s Antibody Portal.
Access NCI-60 cell lines
Our scientists subject each antibody to informative assays and techniques that collectively reveal a comprehensive picture of its characteristics. We provide our approaches, standardized methods, and the complete characterization data (both positive and negative results). Our ready access to the NCI-60 human cancer cell lines makes our program a distinct, if not unique, resource.
Access the Antibody Portal
Immunization process for call-out antibodies
Initial screenings identify potential candidates, further validated in the technique of interest (immunohistochemistry, immunoprecipitation flow cytometry, and other assays). The selected purified antibody is tested, with particular attention to endogenous detection, using Western blotting applications (cell or tissue lysates) and cell-based applications. We collaborate with investigators from both internal (National Institutes of Health) and external institutions (academia).
Characterization process and pipeline
Our scientists produce up to three affinity reagents for each expressed and purified antigen, and one affinity reagent per peptide. We characterize selected reagents using specialized processes.
Our capabilities and specializations
Western blot (including automated Western and single-cell Western)
Surface plasmon resonance
Bio-Layer Interferometry
ELISA
Mass spectrometry immunoassay
Immunohistochemistry
NCI-60 Protein Array
Immunofluorescence
Flow Cytometry
Immunoprecipitation
Need for renewable reagent for the detection of ITGA11 for model glioma studies
