The Pharmacokinetics and Drug Analysis Group performs pharmacokinetic analysis of samples to monitor the concentrations of drugs and their metabolites over time. We analyze samples obtained from clinical trial patients, animal models, or in vitro studies. 

Our team also monitors endogenous biochemical alterations within an overall framework to optimize drug development. 

Analytical chemical methodology expertise 

Our work involves the development and use of analytical chemical methodology. The sophisticated techniques employed require extensive use of high-performance liquid chromatography, mass spectrometry, and gas chromatography, as well as in vitro cell culture and subcellular metabolic tools to complement the biochemical characterizations obtained from in vivo studies.  

Our capabilities and specializations

Analysis of 8-oxo-deoxyguanosine in DNA 

We developed a reliable and novel method for the analysis of 8-oxo-deoxyguanosine in DNA. Our method is used in studies to determine the extent of DNA oxidation caused by a select agent’s ability to generate reactive oxygen species as a biomarker in mechanism-of-action determinations. 

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  • Examining DNA oxidation by reactive oxygen species 
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Analysis of the active metabolites of IPdR 

In collaboration with the Mayo Clinic, we successfully measured plasma metabolite as well as the levels of incorporation of the active metabolite into the DNA of brain tumors in mice treated with IPdR. 

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  • Improving and expanding methods for analyzing the active metabolites of IPdR 

  • Using these methods in IPdR combination studies