The Genome Modification Core provides cutting-edge solutions using various modalities of genome editing to the National Cancer Institute’s Center for Cancer Research and Division of Cancer Epidemiology and Genetics.
The range of services is broad and includes discussing specific strategies; facilitating the generation of transgenic mouse models in collaboration with the Mouse Modeling and Cryopreservation Core; and providing reagents for both single-gene manipulation and pooled genetic screens for knockout, repression, and gene activation.
Broadening understanding of gene editing and generating reagents for the research community
High-throughput genomic technologies and conventional hypothesis-driven research typically uncover aberrations at the genetic level. Genome-editing technologies permit the incorporation of such alterations in various model systems to understand their importance, consequences, and impact.
Email sgrnascorer@nih.gov for questions related to the sgRNA Scorer or dbGuide.
Database for validated guide RNA sequences used in gene-editing experiments
New high-throughput method
The Genome Modification Core developed a high-throughput approach to generate and identify clonally edited populations. Our robust method works across multiple cell lines, including knockout and homology-directed repair strains.
State-of-the-art equipment for genome editing
We maintain three key pieces of equipment that are vital to our day-to-day operation:
- A high-throughput Illumina sequencer to evaluate the editing efficiencies of our genome-editing reagents as well as reagents needed for large-scale genetic screens
- A flow cytometer for sorting single cells into a 384-well plate that enables the generation of hundreds of genetically manipulated clones
- A mammalian electroporation instrument for delivering genome-editing reagents into various cell types, including primary cells that are not immortalized
Our capabilities and specializations
CRISPR-Cas9 genome editing
The Genome Modification Core uses various nucleases and targeted or genome-wide libraries to engineer cells and generate genetically modified mice. We evaluate the editing efficiencies of our genome-editing reagents as well as reagents needed for large-scale genetic screens, generate hundreds of genetically manipulated clones, and deliver genome-editing reagents into varied cell types, including primary cells that are not immortalized.
We also generate genome-editing reagents to genetically modify pre-implantation mouse embryos in collaboration with the Mouse Modeling and Cryopreservation Core.
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Genetic sequencing
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Flow cytometry
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Mammalian electroporation