HPV Operating Procedures

Overview

Image
A pipette with a pink liquid over a well plate

Protection against Human Papillomavirus (HPV) infection after vaccination is believed to be mediated by HPV-specific antibodies. Antibody responses in HPV prophylactic vaccine trials have been assessed using different methods.

The lack of standardized assays, procedures, and reagents accessible to the scientific community has precluded the comparison of different studies evaluating immunogenicity of HPV vaccines.

The HPV Serology Laboratory at the Frederick National Laboratory was established in January 2017 to address some of these needs and advance HPV assay standardization. The HPV Serology Laboratory, now the Serology Laboratory, leads an HPV Serology Standardization initiative in collaboration with the National Cancer Institute and the Bill and Melinda Gates Foundation

We invite you to download and use the following standard operating procedures in your own serology laboratory.

Standard Operating Procedures

Equipment

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HSL EQ 010 Use and Maintenance of the Fisher Scientific Isotemp GDP10 Water Bath Dec 2018
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Laboratory Assays

Document Number and Title Date Updated
Jan 2020 
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HSL LAB 013 Lipid-Mediated pDNA Ripcord Method of Transfection in HEK293TT for VLP Production Dec 2018
HSL LAB 014 HPV VLP Conjugation to Luminex-based Microspheres Dec 2018
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General Laboratory Procedures

Document Number and Title Date Updated
Dec 2018

Note: Procedures should only be used as guidance documents and not substituted for your own institute's/organization's/government's policies.