The Imaging Mass Cytometry Laboratory provides cancer researchers access to the technology to enable pilot studies, collection of preliminary data, and investigations that complement ongoing research. 

Submissions will be assessed based on the scientific plan, the need for imaging mass cytometry (IMC) to advance the research, and the potential for broader cancer research impact. Requests with IMC analyses as a critical component of an NCI-supported project or future NCI proposal, will be prioritized. Priority will also be given to requests from under-resourced institutions without access to IMC or other multiplexed imaging approaches. 

Selected applicants will be required to agree to the IMC Data Sharing Policy, to ensure all data collected by the Imaging Mass Cytometry Laboratory follows the NIH Data Management and Sharing (DMS) policy.

Accepted projects will be incorporated into laboratory workflows based on resource availability. Ensuring projects are completed in a timely manner will require close coordination with the Imaging Mass Cytometry Laboratory project manager, as well as a prompt shipment of specimens and reagents.

Please submit this form to request a service. All investigators with a cancer-focused project are welcome to submit.

Contact
(If relevant, having an NCI grant is not a requirement)

Project-specific details

Provide a brief summary of the overarching research project, including background, significance, and goals.

500 word limit, suggest following Specific Aims guidance in the NIH application guide. Strong requests will have a well-supported scientific premise, clear goals, and potential to impact cancer research beyond the immediate research area.
How will imaging mass cytometry (IMC) specifically enable the planned research direction? What capabilities does this laboratory provide that you do not otherwise have access to?

300 word limit. Strong requests will delineate what this approach can enable compared to other available approaches; priority will be given to submitters without access to these technologies.
For the IMC component, define the experimental design and project milestones.

300 word limit. Plans could include evaluating the antibody panel on control tissues, testing the panel in x number of samples, successful measurement of x more proteins beyond the current method, etc. Strong requests will have clear milestones that define the scope of the IMC project.
Describe how the IMC analyses will support an NCI-supported project or future NCI application?

300 word limit. Strong requests will demonstrate clear importance of the analyses to a current or future NCI-funded cancer research project.
Data sharing
Are you willing to share the images and analysis data in a publicly accessible repository? Will you allow the IMC Laboratory to publicly share the antibody panels developed for your project?

Priority will be given to submitters willing to share data and antibody panels.

Request specifications and technical details

Resources/expertise required
Specify the technical expertise and resources required to conduct the project, that are expected to be provided by the Imaging Mass Cytometry Laboratory.
Sample Source
What is the source of samples that you will send?
Tissue type
What type of tissues would you send to the laboratory?
Antibodies
Are you able to provide the IMC laboratory with the metal labelled antibodies required for testing?
May include inquiries regarding additional technical requirements or services not listed as options above.
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Contact Christine Newkirk, project manager, with any questions.