The Imaging Mass Cytometry Laboratory provides cancer researchers with high-dimensional imaging analysis of tumor tissue samples at subcellular resolution.
We work with researchers interested in studying the tumor microenvironment, tumor-stromal cells interactions, and the roles of different subpopulations of immune cells in tumor progression and suppression in animal models and cancer patients' samples.
The laboratory is equipped with a Fluidigm Hyperion Imaging System that performs high-dimensional proteomics analysis of fresh-frozen tissues, tissue microarrays, smeared and formalin-fixed cells, paraffin-embedded tissue.
With this technology, we use metal-labeled antibodies to stain the specimens on glass slides and obtain high-dimensional images of up to 40 protein markers simultaneously. This allows researchers to interrogate the protein markers expression in the nucleus and cytoplasm, as well as on the cell membrane.
In addition to the tissue slides analysis, the laboratory has implemented this technology for multiplex proteomics characterization of rare circulating tumor cells (CTCs) isolated from cancer patients’ blood samples. This helps to better understand the role of CTCs in the metastatic spread of a disease and to study drug resistance and mutational switchover.
We work with researchers to design customized metal-label antibody panels, validate those panels, and optimize the staining protocols. The Imaging Mass Cytometry Laboratory also maintains a state-of-the-art data analysis pipeline for quantitative data analysis of high-dimensional, single-cell data.