Available Strain Details

Strain Information
Common Strain Name: Pax7(CreERp) Pricing and Ordering Information
Strain Nomenclature: B6;129-Pax7tm1(cre/Esr1*)Cklr/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): D

Strain Description: No phenotype for the CreER allele alone.
Mutation Information
Mutation Type: Targeted
Gene Name: ires-CreER(tm)
Gene Symbol: ires-CreER(tm)
Transgene Name:
Transgene Symbol:
Promotor Name: Pax7 3' untranslated region (between stop codon and poly A signal) mouse
Promotor Symbol: Pax7
Current Genetic Background: B6;129
Approx. Generation: 4
Organ Site: Muscle, Brain, Pituitary
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote male x wildtype C57BL/6 female. Per Dr. Charles Keller, the Donating Investigator, "CreER must ALWAYS come from the sire only to prevent somatic mosaicism; experimentals are NEVER homozygous". Average litter size is 5-8.
Genotyping Information: Protocol 1: Allele: Pax7<tm1(cre/Esr1*)Cklr>
Donating Investigator: Dr. Charles Keller

Key Reference: Koichi Nishijo, Tohru Hosoyama, Christopher R.R. Bjornson, Beverly Schaffer, Ali N. Bahadur, Mark S. Hansen, Mary C. Blandford, Amanda T. McCleish, Brian P. Rubin, Jonathan A. Epstein, Thomas A. Rando, Mario R. Capecchi, Charles Keller. Biomarker System for studying muscle, stem cells and cancer in vivo. The FASEB Journal, 23(8):2681-90, August 2009 [Pubmed UID 19332644] [Pubmed Abstract]

Related Reference: Tohru Hosoyama, Koichi Nishijo, Melinda M. Garcia, Beverly S. Schaffer, Sachiko Ohshima-Hosoyama, Suresh I. Prajapati, Michael D. Davis, Wilmon F. Grant, Bernd W. Scheithauer, Daniel L. Marks, Brian P. Rubin, Charles Keller. A Postnatal Pax7+ Progenitor Gives Rise to Pituitary Adenomas. Genes & Cancer, epub May 7, 2010, PMID: 20811506 [Pubmed Abstract]

Suresh I. Prajapati, Carlo O. Martinez, Jinu Abraham, Amanda T. McCleish, Joel E. Michalek, Brian P. Rubin, Paula K. Shireman, Charles Keller. Crimson Carrier, A Long-Acting Contrast Agent for In Vivo Near-Infrared Imaging of Injured and Diseased Muscle. Muscle and Nerve, epub April 14, 2010 [NIHMSID #207740], PMID: 20544935 [Pubmed Abstract]

Sponsor: 1 K08 CA 90438-01 Keller (PI), NIH/NCI