Strain Protocols

Strain Information
Strain Code: 01XP5
Strain Name: STOCK Ptentm1.1Whs/Nci
Gene Symbol: PTEN
Protocol Information
Allele: Pten<tm1.1Whs>/Nci
Protocol Number: 1
Introductory Comment:

Primers

Y602 : 5'-ACA GGC CCA TGT TGT ATA GAC A-3'
Y603 : 5'-GGA GAC TGA AGG CAA TCC TGC A-3'
Y604 : 5'-ACA GGC CCA TGT TGT ATA GAC A-3'
Y605 : 5'-CGC CTA CCG GTG GAT GTG GAA T-3'

Product Sizes

Primer Combination Product Size
Y602 / Y603 291 bp    Wild type
Y604 / Y604 380 bp    Mutant

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2
2.5mM dNTP Mix 0.2mM
100 µMY602 0.3 µM
100 µMY603 0.3 µM
100 µMY604 0.5 µM
100 µMY605 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.