Strain Protocols

Strain Information
Strain Code: 01XNA
Strain Name: FVB-Tg(Sprr2f-cre)1Dcas/Nci
Gene Symbol: Cre
Protocol Information
Allele: Tg(Sprr2f-cre)1Dcas
Protocol Number: 1
Introductory Comment:

Primers

NA1 : 5'-GGT ACA CAC GTC CTG GAA TAC-3'
NA2 : 5'-TTC CCA TTC TAA ACA ACA CCC TGA A-3'

Product Sizes

Primer Combination Product Size
NA1 / NA2 Tg    182 bp

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2mM
2.5mM dNTP Mix 0.2mM
100 µMNA1 0.5 µM
100 µMNA2 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.