Strain Protocols

Strain Information
Strain Code: 01XG1
Strain Name: FVB.129-Nkx3-1tm1Mms /Nci
Gene Symbol: Nkx3.1
Protocol Information
Allele: Nkx3.1<tm1Mms>
Protocol Number: 1
Introductory Comment:

Primers

N005 : 5'-Gcc AAc cTG ccT cAA TcA cTA AGG-3'
N006 : 5'-TTc cAc ATA cAc TTc ATT cTc AGT-3'
N007 : 5'-Gcc AcA GTG GcT GAT GTc AAG GAG TcG G-3'

Product Sizes

Primer Combination Product Size
N005 / N007 300 bp    Knockout
N005 / N006 500 bp    Wildtype

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.5 mM
2.5mM dNTP Mix 0.2 mM
100 µMN005 0.33 µM
100 µMN006 0.33 µM
100 µMN007 0.33 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.