ES Cell Details

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ES Cell: miR-100 (TRE-tight/miR-30)
Stock Number: M000272
Description: This ES cell contains a mature miR-100 in miR-30 context controlled by TRE-tight promoter.
Oligonucleotide sequence of mature miR-100 in miR-30 context. TGCTGTTGACAGTGAGCGAACAAGTTCGGATCTACGGGTTTAGTGAAGCCACAGATGTAAACCCGTAGATCCGAACTTGTGTGCCTACTGCCTCGGA
Validation of ES cells expressing miR-100
- dox
+ dox
Flow cytometric analysis of GFP activity without green fluorescence and then with green fluorescence for the given ES cell
A) Flow cytometric analysis of GFP activity in an ES cell line expressing miR-100 in the TRE-tight/miR-30 in the absence of doxycycline (control: left image), and in the presence of doxycycline (right image), plotting cell count (Y-axis) as a function of relative fluorescence (X-axis). GFP expression is demonstrated by the green curve verses the non-GFP-expressing cell population shown in pink. Selected ES cells were grown in 24-well plates in the absence or presence of doxycycline (1 μg/ml) for 48 hours.
Fold induction of miR expression for miR-100Image shows the Tet-inducible expression of let-7c for the given ES Cell
B) Tet-inducible expression of miR-100 as determined by RT-qPCR (the fold induction of miR-100 expression was calculated following treatment with doxycycline).