Available Strain Details

Strain Information
Common Strain Name: hGFAP-Cre Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Tg(GFAP-cre)8Gtm/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: Transgenic mouse expressing Cre recombinase under control of the astrocyte-specific human GFAP promoter (Bajenaru et al., 2002). The transgene construct included an internal ribosome entry site (IRES) to allow for co-expression of Cre and beta-galactosidase (LacZ) from the same transcript. Astrocyte-specific expression of Cre and LacZ was confirmed by immunohistochemistry. Beta-galactosidase activity was first detected in the subventricular zone by embryonic day 14.5. GFAP-Cre mice are phenotypically normal. This strain has been used for astrocyte-specific inactivation of several cancer-related genes, including Nf1 (Bajenaru et al., 2002, 2003), Tsc1 (Uhlmann et al., 2002), and Pten (Fraser et al., 2004).
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Cre recombinase
Transgene Symbol: Cre
Promotor Name: GFAP, human
Promotor Symbol: GFAP
Current Genetic Background: B6.Cg
Approx. Generation: 28
Organ Site: Brain
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 5-6.
Genotyping Information: Protocol 1: Allele: Tg(GFAP-cre)8Gtm
Donating Investigator: Dr. David H. Gutmann

Key Reference: Bajenaru ML, Zhu Y, Hedrick NM, Donahoe J, Parada LF, Gutmann DH. Astrocyte-specific inactivation of the neurofibromatosis 1 Gene (Nf1) is insufficient for astrocytoma formation. Mol. Cell. Biol. 22, 5100-5113, 2002. PMID: 12077339 [Pubmed Abstract]

Related Reference: Bajenaru ML, Hernandez MR, Perry A, Zhu Y, Parada LF, Garbow JR, Gutmann DH. Optic nerve glioma in mice requires astrocyte Nf1 gene inactivation and Nf1 brain heterozygosity. Cancer Res. 63, 8573-8577, 2003. PMID: 14695164 [Pubmed Abstract]

Bajenaru ML, Garbow JR, Perry A, Hernandez MR, Gutmann DH. Natural history of neurofibromatosis 1-associated optic nerve glioma in mice. Ann Neurol. 57, 119-127, 2005. PMID: 15622533 [Pubmed Abstract]

Fraser MM, Zhu X, Kwon CH, Uhlmann EJ, Gutmann DH, Baker SJ. Pten loss causes hypertrophy and increased proliferation of astrocytes in vivo. Cancer Res. 64, 7773-7779, 2004. PMID: 15520182 [Pubmed Abstract]

Sponsor: The National Institutes of Health, NIH NS36996