Available Strain Details

Strain Information
Common Strain Name: Mdm-2puro Pricing and Ordering Information
Strain Nomenclature: 129-Mdm2tm1Mep/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: The mdm2puro allele is a hypomorphic and conditional allele that can be converted to either a fully functional conditional allele or a null allele by the Cre recombinase. Mice expressing mdm2puro (Tac125) allele and one known null allele of mdm2 have reduced levels of expression of the oncogene mdm2 and, as a consequence, are small, radiosensitive and lymphopenic. All phenotypes are dependent on p53. Although the transcriptional activation function of p53 is increased in many tissues, spontaneous, p53-dependent apoptosis occurs in only a subset of these tissues. These mice will be valuable for identifying the regulatory steps that control p53 function in tissues.
Mutation Information
Mutation Type: Targeted
Gene Name: Murine double minute 2
Gene Symbol: Mdm2
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: 129
Approx. Generation:
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is 129S1/SvImJ/Cr females x homozgyous males or 129S1/SvImJ/Cr females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as homozygotes.
Genotyping Information: Protocol 1: Allele: Mdm2<tm1Mep>
Donating Investigator: Dr. Mary Ellen Perry

Key Reference: Mendrysa SM, McElwee MK, Michalowski J, O'Leary KA, Young KM, and Perry ME. 2003 mdm2 Is Critical for Inhibition of p53 during Lymphopoiesis and the Response to Ionizing Irradiation. Molecular and Cellular Biology 23:462-472. PMID: 12509446 [Pubmed Abstract]