Available Strain Details

Strain Information
Common Strain Name: PML null Pricing and Ordering Information
Strain Nomenclature: 129/Sv-Pmltm1Ppp/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3

Strain Description: Mice homozygous for this mutation (-/-) have a reduced number of circulating granulocytes, basophils and eosinophils, and a reduction in the number of circulating myeloid cells. There is no increase in spontaneous tumors over wildtype (+/+) littermates during the first year of life, but -/- mice develop more tumors than +/+ mice when exposed to dimethylbenzanthracene (DMBA) followed by 12-O-tetradecanoylphorbol-13-acetate (TPA). Retinoic acid has little growth suppressive activity on embryonic fibroblasts (MEFs) derived from -/- mice. MEFs derived from -/- mice grow faster than those derived from +/+ mice and are markedly better at forming colonies. According to the Donating Investigator, Dr. Pier Paolo Pandolfi, this strain is susceptible to staph infections. No special husbandry precautions are used to prevent them provided normal safety measures are utilized.
Mutation Information
Mutation Type: Targeted
Gene Name: promyelocytic leukemia
Gene Symbol: PML
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: 129/Sv
Approx. Generation: 14
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is 129S1/SvlmJ/Cr females x homozgyous males or 129S1/SvlmJ/Cr females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as homozygotes.
Genotyping Information: Protocol 1: Allele: Pml<tm1Ppp>
Donating Investigator: Dr. Pier Paolo Pandolfi

Key Reference: Wang ZG, Delva L, Gaboli M, Rivi R, Giorgio M, Cordon-Cardo C, Grosveld F, Pandolfi PP. Role of PML in cell growth and the retinoic acid pathway. Science. 1998 279:1547-51 PMID: 9488655 [Pubmed Abstract]

Related Reference: Wang ZG, Ruggero D, Ronchetti S, Zhong S, Gaboli M, Rivi R, Pandolfi PP. PML is essential for multiple apoptotic pathways. Nat Genet. 1998 20:266-72 PMID: 9806545 [Pubmed Abstract]