Available Strain Details

Strain Information
Common Strain Name: Nkx3.1-Cre Pricing and Ordering Information
Strain Nomenclature: B6;129S-Nkx3-1tm3(cre)Mms/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: This is a knock-in of Cre into the Nkx3.1 locus, placing it under the transcriptional control of the Nkx3.1 promoter, and creating a null allele. Cre recombinase activity is detected in nearly all prostate epithelial cells (luminal and basal) starting from before birth.
Mutation Information
Mutation Type: Targeted
Gene Name: NK-3 transcription factor, locus 1 (Drosophilia)
Gene Symbol: Nkx3-1
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129S
Approx. Generation: Homo
Organ Site: Prostate
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is homozygous x homozygous. When maintained as a live colony, breeder pairs were distributed as homozygotes. Homozygous males should be bred at a young age (within the first 4-5 months). Occasionally, if not bred early on, they do not mate; some homozygous males never breed. If there is a breeding cage that has not had pups for 2 months, the male should be removed and a new one put in. Once they start breeding they do fine. Average litter size is 6-8.
Genotyping Information: Protocol 1: Allele: Nkx3-1<tm3(cre)Mms>
Donating Investigator: Dr. Michael Shen

Key Reference: Lin, Y., Lui, G., Zhang, Y., Hu, Y.-P., Yu, K., Lin, C., McKeehan, K., Xuan, J. W., Ornitz, D., Shen, M. M., Greenberg, N., McKeehan, W. L., and Wang, F. (2007). Fibroblast growth factor receptor 2 tyrosine kinase is required for prostatic morphogenesis and acquisition of strict androgen dependency for adult tissue homeostasis. Development 134: 723-734. PMID: 17215304 [Pubmed Abstract]

Related Reference: Zhang, Y., Zhang, J., Lin, Y., Lan, Y., Lin, C., Xuan, J. W., Shen, M. M., McKeehan, W. L., Greenberg, N. M., and Wang, F. (2008). Role of epithelial cell fibroblast growth factor receptor substrate 2alpha in prostate development, regeneration and tumorigenesis. Development 135: 775-784. PMID: 18184727 [Pubmed Abstract]

Thomsen, M. K., Butler, C. M., Shen, M. M., and Swain, A. (2008). Sox9 is required for prostate development. Dev. Biol. 316: 302-311. PMID: 18325490 [Pubmed Abstract]

Placencio, V. R., Sharif-Afshar, A.-R., Li, X., Huang, H., Uwamariya, C., Neilson, E. G., Shen, M. M., Matusik, R. J., Hayward, S. W., and Bhowmick, N. A. (2008). Stromal Transforming Growth Factor-beta signaling mediates prostatic response to androgen ablation by paracrine Wnt activity. Cancer Res. 68: 4709-4718. PMID: 18559517 [Pubmed Abstract]

Sponsor: U01 CA084294 (NCI Mouse Models of Human Cancers Consortium), R01 CA115985