Available Strain Details

Strain Information
Common Strain Name: N-ras null, Nras KO Pricing and Ordering Information
Strain Nomenclature: B6;129P2-Nrastm1Rak/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B1

Strain Description: This strain carries a null mutation in N-ras, one of three highly homologous and widely expressed members of the ras family. Mice homozygous for this mutation develop normally (Umanoff et al., 1995). When combined with K-ras heterozygosity, mice exhibit abnormal hematopoietic development and die between days 10 and 12 of embryogenesis (Johnson et al., 1997).
Mutation Information
Mutation Type: Targeted
Gene Name: neuroblastoma ras oncogene
Gene Symbol: Nras
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129
Approx. Generation: 15
Organ Site: hematopoietic
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. SPERM: Sperm was obtained from heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female).
Genotyping Information: Protocol 1: Allele: Nras<tm1Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Umanoff H, Edelmann W, Pellicer A, Kucherlapati R. The murine N-ras gene is not essential for growth and development. Proc Natl Acad Sci USA 92, 1709-1713, 1995. PMID: 7878045 [Pubmed Abstract]

Related Reference: Johnson L, Greenbaum D, Cichowski K, Mercer K, Murphy E, Schmitt E, Bronson RT, Umanoff H, Edelmann W, Kucherlapati R, Jacks T. K-ras is an essential gene in the mouse with partial functional overlap with N-ras. Genes Dev 11, 2468-2481, 1997. PMID: 9334313 [Pubmed Abstract]

Sponsor: NIH HG00380, American Cancer Society CN-132, NCI CA13330, NIH T32NS07183, Deutsche Forschungsgemeinschaft