Available Strain Details

Strain Information
Common Strain Name: Apc CKO Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Apctm2Rak/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B1

Strain Description: This strain carries a conditional allele of Apc with two LoxP sites flanking exon14 (a FRT-flanked neomycin resistance cassette was removed by FLP-mediated recombination). Homozygous mice are phenotypically normal and fertile. These animals can be used for tissue-specific ablation of Apc function. Deletion of exon 14 by cre-mediated recombination leads to expression of a truncated Apc protein of 605 amino acid residues (aa), of which the first 580 aa correspond to the normal protein.
Mutation Information
Mutation Type: Targeted
Gene Name: Adenomatosis polyposis coli
Gene Symbol: Apc
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.Cg
Approx. Generation: 24
Organ Site: Various
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is homozygous females x homozygous males. SPERM: Sperm was obtained from homozygous males. When maintained as a live colony, breeder pairs were distributed as homozygotes. Average litter size is 7.
Genotyping Information: Protocol 1: Allele: Apc<tm2Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Kuraguchi M, Wang XP, Bronson RT, Rothenberg R, Ohene-Baah NY, Lund JJ, Kucherlapati M, Maas RL, Kucherlapati R. Adenomatous polyposis coli (Apc) is required for normal development of skin and thymus. PLoS Genetics 2, 1362-1374, 2006. PMID 17002498 [Pubmed Abstract]