The Neutrophil Monitoring Laboratory performs testing to assess neutrophil dysfunction in patients with various primary immunodeficiencies.
We develop state-of-the-art assays to evaluate a variety of neutrophil functions such as reactive oxygen species production, chemotaxis, cell shape change and spreading, exocytosis, and alterations in surface antigen expression.
The laboratory has developed and performs high complexity, CLIA-certified, assays that are used to diagnose patients with:
Chronic granulomatous disease, a genetic immunodeficiency characterized by recurrent bacterial and fungal infections caused by a defect in reactive oxygen species production
Leukocyte adhesion deficiency, a defect in the ability of neutrophils to migrate to sites of infection.
We employ several multiplex analysis platforms to monitor for the levels of inflammatory cytokines and other biomarkers in biological specimens or culture supernatant fluids.
The Neutrophil Monitoring Laboratory provides clinical and basic research in support of the Laboratory of Clinical Immunology and Microbiology at the National Institute of Allergy and Infectious Diseases. We maintain an extensive repository of patient plasma, cryopreserved peripheral blood mononuclear cells, Epstein-Barr virus-transformed B-cell lines, dermal fibroblasts, DNA, and RNA that represent a valuable resource for investigators.