Technical Service Agreement (TSA)

Overview

Frederick National Laboratory for Cancer Research (FNLCR) scientists provide services and solutions to collaborators through the Technical Services Program, whose portfolio includes more than 200 collaborations with more than 80 partners. FNLCR’s unique offerings cover HIV and SIV services; KRAS/p53 mouse models and cell lines for PDAC and NSCLC with assessment of drug toxicity, efficacy, tolerance, survival, and pharmacokinetics; 1,520 mouse embryonic cell lines with most known mouse miRNAs; HLA genotyping; expression profiling for human B-cell lymphoma on the NanoString platform.

For more information on our Technical Services, contact:

Sanya Fanous Whitaker, Ph.D.
Senior Partnership Alliance Manager
Sanya.Whitaker@nih.gov
(301) 846-6058

HIV/SIV Services

Service

Description

Contact

FNLCR Technical Service Number

qPCR SIV Viral Load Analysis

  • qPCR and qRT-PCR for SIV replication and expression
  • Coverage of isolates used most commonly in SIV experimental studies
  • Plasma analyzed at standard (15 copies/mL) or ultrasensitive (1.6 copies/mL) detection levels

Jeff Lifson, M.D.,
lifsonj@mail.nih.gov

 

ACVP-02
ACVP-13

Hybrid RT/Digital SIV qPCR Viral Load Analysis

  • Samples (cell pellets or tissue) prepared as necessary
  • Full protocol for both RNA and DNA determinations including cell equivalents

Jeff Lifson, M.D.,
lifsonj@mail.nih.gov

 

ACVP-06
ACVP-08

HIV/SIV Single Genome Amplification/ cDNA Synthesis

  • Viral DNA/RNA extraction from cell-free or cell-associated samples
  • Single copy PCR amplification of entire viral gene of interest
  • Comprehensive sequence analysis included

Brandon Keele, Ph.D.,
keelebf@mail.nih.gov

 

ACVP-05

Laboratory Animal Services

Service

Description

Contact

FNLCR Technical Service Number

Use of Mouse Embryonic Stem Cells expressing miRNAs

  • Tetracycline-inducible novel mouse embryonic cell lines
  • 1,520 cell lines available, with most known mouse miRNAs

Debra Fitzgerald,
fitzgeraldd@mail.nih.gov

 

LASP-01

In Vitro Cell Cytotoxicity Evaluation

  • Dose-response to candidate compounds measured at 6 escalating concentrations
  • Available in Kras/p53 PDAC or Kras/p53 NSCLC derived cells

Serguei Kozlov, Ph.D.,
kozlovse@mail.nih.gov

LASP-02
LASP-08

Pharmacokinetic Evaluation in Kras/p53 PDAC mice

  • Five time points plus vehicle control
  • Collection of plasma/serum and multiple tissue samples for PK
  • Detailed pathology assessment of H&E-stained tissues at endpoint

Serguei Kozlov, Ph.D.,
kozlovse@mail.nih.gov

LASP-03

Tolerance Evaluation of Experimental Compound in Kras/p53 PDAC mice

  • One or two dosing arms plus a vehicle control arm
  • Includes full toxicology report
  • Includes cryopreserved tissues and blood plasma

Serguei Kozlov, Ph.D.,
kozlovse@mail.nih.gov

 

LASP-04

Time Course Assessment of Tumor Response to Therapeutics

  • Four arm study
  • Tumor monitoring by longitudinal imaging (MRI or ultrasound)
  • Includes biomarker evaluation, cryopreserved tissues and plasma
  • Available in Kras/p53 PDAC or Kras/p53 NSCLC mice

Serguei Kozlov, Ph.D.,
kozlovse@mail.nih.gov

LASP-05
LASP-09

Assessment of Drug Efficacy in a Survival Study

  • Available in three, four, or five arm study format
  • Includes cryopreserved tissues and blood plasma
  • Includes quantitative assessment of up to five informative biomarkers by immunohistochemistry 
  • Available in Kras/p53 PDAC or Kras/p53 NSCLC mice

Serguei Kozlov, Ph.D.,
kozlovse@mail.nih.gov

 

LASP-06
LASP-07
LASP-10
LASP-11

 

Other Services

Service

Description

Contact

FNLCR Technical Service Number

Class I & II HLA 4-digit typing

  • Ten HLA genes amplified and HLA genotype assigned with 4-digit resolution

Pat Martin, M.D.,
Maureen.Martin@nih.gov

BSP-01

mRNA isolation and expression analysis in human B-cell lymphoma samples

  • 20-gene panel to identify Activated B-cell (ABC) and Germinal Center B-cell (GCB) subtypes of Diffuse Large B-cell Lymphomas (DLBCL) using the NanoString platform under CLIA conditions in formalin-fixed paraffin-embedded (FFPE) samples

Daniel Soppet, Ph.D.,
soppetdr@mail.nih.gov

 

CRTP-13