Available Strain Details

Strain Information
Common Strain Name: Nras-Q61R L/L Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Nrastm1.1Nesh Cdkn2atm2.1Nesh Tg(Tyr-cre/ERT2)13Bo/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: When treated topically with 4-hydroxytamoxifen to induce melanocyte-specific CRE activation, these mice spontaneously develop cutaneous melanoma
Mutation Information
Mutation Type: Targeted
Gene Name: neuroblastoma ras oncogene
Gene Symbol: Nras
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.Cg
Approx. Generation:
Organ Site: skin
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is homozygous males x C57BL/6 females. When maintained as a live colony, the donor maintained homozgyous colonies. Average litter size is 6.
Genotyping Information: Protocol 1: Allele: Cdkn2a<tm2.1Nesh>
Protocol 1: Allele: Nras<tm1.1Nesh>
Donating Investigator: Dr. Norman Sharpless

Key Reference: Mutation-specific RAS oncogenicity explains NRAS codon 61 selection in melanoma, Burd et al, Cancer Discov. 2014 Dec;4(12):1418-29. doi: 10.1158/2159-8290.CD-14-0729. Epub 2014 Sep 24. PMID:25252692 [Pubmed Abstract]

Related Reference: For p16f allele: Somatic p16(INK4a) loss accelerates melanomagenesis, Monahan et al, Oncogene. 2010 Oct 28;29(43):5809-17. doi: 10.1038/onc.2010.314. Epub 2010 Aug 9., PMID:20697345 [Pubmed Abstract]

For Tyr::CreER(T2) allele: Characterization of melanocyte-specific inducible Cre recombinase transgenic mice. Bosenberg et al, Genesis. 2006 May;44(5):262-7.PMID:16676322 [Pubmed Abstract]

Sponsor: NIH R00AG036817, NIH R01CA163896, NIH T32ES007126, NIH R01CA185353