Available Strain Details

Strain Information
Common Strain Name: CAGGS-SB10 (Sleeping Beauty) Pricing and Ordering Information
Strain Nomenclature: FVB-Tg(ACTB-SB10)1Dla/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): C1

Strain Description: CAGGS-SB10 mice express Sleeping Beauty SB10 transposase under control of the chimeric, ubiquitously active CAGGS promoter. This strain, in combination with the two transposon concatemer strains (68 T2/Onc, 76 T2/Onc), constitutes the Sleeping Beauty mutagenesis system that allows for unbiased, forward genetic screens for cancer genes in vivo.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: mouse (sleeping beauty)
Transgene Symbol: SB10
Promotor Name: CAGGS (chimeric)
Promotor Symbol: CAGGS
Current Genetic Background: FVB
Approx. Generation: 13
Organ Site: Various
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype FVB (male or female). Average litter size is 8-10.
Genotyping Information: Protocol 1: Allele: Tg(ACTB-SB10)1Dla
Donating Investigator: David Largaespada

Key Reference: Dupuy AJ, Fritz S, Largaespada DA. Transposition and gene disruption in the male germline of the mouse. Genesis 30, 82-88, 2001. PMID: 11416868. [Pubmed Abstract]

Related Reference: Collier LA, Carlson CM, Ravimohan S, Dupuy AJ, Largaespada DA. Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse. Nature 436, 272-276, 2005. PMID: 16015333. [Pubmed Abstract]

Sponsor: NIH-NCI, U01 (CA84221, Years 6-10), 2/15/03 - 3/31/09, NIH-NIDA, R01 (DA014764), 2/15/03 - 11/30/05