Available Strain Details

Strain Information
Strain Number: 01XMD Register Interest
Common Strain Name: Mdm2/p53MdmX triple KO
Strain Nomenclature: B6;129S-Mdm4GT(VICTR20)7Lex Mdm2tm1BayTrp53tm1Brd/Nci
Animal State:
Release Category (Required for MTA form): B3,C1

Strain Description: Absence of Mdm2 and/or MdmX induces embryonic lethality that can be rescued fully by co-deletion of p53. These mice present tumor at the same rate and incidence as p53-null mice, and are useful in dissecting p53-independent roles for Mdm proteins in development and homeostasis.
Mutation Information
Mutation Type: Targeted
Gene Name: Mouse Double Minute 4
Gene Symbol: Mdm4
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129S7
Approx. Generation:
Organ Site: all
Additional Information
Husbandry: Cryopreservation mating scheme is homozygous females x homozygous males. When matintained as a live colony average litter size is 4-5. Mice will present with tumors at 7 months.
Genotyping Information: Protocol 1: Allele: Gt(VICTR20)7Lex
Protocol 1: Allele: Mdm2<tm1Bay>
Protocol 1: Allele: Trp53<tm1Brd>/Nci
Donating Investigator: Dr. Stephen Jones

Key Reference: Steinman HA1, Sluss HK, Sands AT, Pihan G, Jones SN. Absence of p21 partially rescues Mdm4 loss and uncovers an antiproliferative effect of Mdm4 on cell growth. Oncogene. 2004 Jan 8;23(1):303-6. PMID: 14712235 [Pubmed Abstract]

Related Reference: Steinman HA1, Hoover KM, Keeler ML, Sands AT, Jones SN. Rescue of Mdm4-deficient mice by Mdm2 reveals functional overlap of Mdm2 and Mdm4 in development. Oncogene. 2005 Nov 24;24(53):7935-40. PMID: 16027727 [Pubmed Abstract]

Matijasevic Z1, Steinman HA, Hoover K, Jones SN. MdmX promotes bipolar mitosis to suppress transformation and tumorigenesis in p53-deficient cells and mice.Mol Cell Biol. 2008 Feb;28(4):1265-73. Epub 2007 Nov 26. PMID: 18039860 [Pubmed Abstract]

Gannon HS1, Woda BA, Jones SN. ATM phosphorylation of Mdm2 Ser394 regulates the amplitude and duration of the DNA damage response in mice. Cancer Cell. 2012 May 15;21(5):668-79. doi: 10.1016/j.ccr.2012.04.011. PMID: 22624716 [Pubmed Abstract]

Sponsor: NIH CA77735