Available Strain Details

Strain Information
Common Strain Name: E2f4 Pricing and Ordering Information
Strain Nomenclature: B6;129S-E2f4tm1Lees/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3

Strain Description: E2f4 is essential for normal development. E2f4 null mice died of an increased susceptibility to opportunistic infections that appeared to result from craniofacial defects. They also display erythroid abnormalities that arise from a cell autonomous defect in late stage maturation. Additional studies indicate that E2f4 plays a role in adipocyte development and also that loss of E2f4 suppresses tumorigenesis in mice heterozygous for a mutation in the tumor suppressor gene pRb. The mechanism for this tumor suppression appears to be different from that in the case of E2f3 loss.
Mutation Information
Mutation Type: Targeted
Gene Name: E2f transcription factor 4
Gene Symbol: E2f4
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129S
Approx. Generation: 5
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). The majority of -/- animals die within 3 to 4 days and more than 85% fail to reach weaning age. E2f4 -/- mice that survive are highly susceptible to opportunistic infections.
Genotyping Information: Protocol 1: Allele: E2f4<tm1Lees>
Donating Investigator: Dr. Jacqueline Lees

Key Reference: Humbert P O, Rogers C, Ganiatsas S, Landsberg RL, Trimarchi JM, Dandapani S, Brugnara C, Erdman S, Schenzel M, Bronson R, and Lees J. 2000 E2f3 is essential for Normal Erythrocyte Maturation and Neonatal Viability. 2000 Molecular Cell 6: 281-291. PMID: 10983976 [Pubmed Abstract]