Strain Protocols

Strain Information
Strain Code: 01XMC
Strain Name: B6;129S7-Mdm2tm1Snj/Nci
Gene Symbol: Mdm2
Protocol Information
Allele: Mdm2<tm1Snj>/Nci
Protocol Number: 1
Introductory Comment:

Primers

Y673 : 5'-GGT CTT CCC ATT TAT GTA TGT-3'
Y697 : 5'-AAG AGT CTG TAT CGC TTT CT-3'

Product Sizes

Primer Combination Product Size
Y673 / Y697 598 bp    Wild type
Y673 / Y697 238 bp    Floxed allele

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2
2.5mM dNTP Mix 0.2mM
100 µMY673 0.5 µM
100 µMY697 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
55ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.