Strain Protocols

Strain Information
Strain Code: 01XM8
Strain Name: FVB/N-Tg(KRT5-tTA)1216Glk/Nci
Gene Symbol: KRT5
Protocol Information
Allele: Tg(KRT5-tTA)1216Glk
Protocol Number: 1
Introductory Comment:

Primers

y01 : 5'-CTC GCC CAG AAG CTA GGT GT-3'
y02 : 5'-CCA TCG CGA TGA CTT AGT-3'

Product Sizes

Primer Combination Product Size
y01 / y02 200 bp    transgene

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2
2.5mM dNTP Mix 0.2mM
100 µMy01 1 µM
100 µMy02 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.