Strain Protocols

Strain Information
Strain Code: 01XJC
Strain Name: B6.129S7-Tg(Mdm2)1Snj/Nci
Gene Symbol: Mdm2
Protocol Information
Allele: Tg(Mdm2)1Snj/Nci
Protocol Number: 1
Introductory Comment:

Primers

Y679 : 5'-gag aca ata acc ctg ata aat gct tc-3'
Y680 : 5'-tcc gat cgt tgt cag agg taa gtt-3'
C003 : 5'-cta ggc cac aga att gaa aga tct-3'
C004 : 5'-gta ggt gga aat tct agc atc atc c-3'

Product Sizes

Primer Combination Product Size
Y679 / Y680 480 bp    Transgene
C003 / C003 325 bp    Internal control

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2
2.5mM dNTP Mix 0.2mM
100 µMY679 0.5 µM
100 µMY680 0.5 µM
100 µMC003 0.5 µM
100 µMC004 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.