Strain Protocols

Strain Information
Strain Code: 01XJ3
Strain Name: FVB-Tg(ACTB-ALPP)03Jrst/Nci
Gene Symbol: PLAP
Protocol Information
Allele: Tg(ACTB-ALPP)03Jrst
Protocol Number: 2
Introductory Comment:

Primers

P017 : 5'-GGA TGG TcT ccA TcT ccT GA-3'
P018 : 5'-AcA TcT GGA cAc TGG GcA TAG-3'

Product Sizes

Primer Combination Product Size
P017 / P018 146 bp    Transgene

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMP017 0.5 µM
100 µMP018 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
0ºC
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.