Strain Protocols

Strain Information
Strain Code: 01XGL
Strain Name: B6.Cg-Tg(ED-L2-cre)267Jkat/Nci
Gene Symbol: Cre
Protocol Information
Allele: Tg(ED-L2-cre)267Jkat
Protocol Number: 1
Introductory Comment: For this strain, we assay for the presence of the Cre transgene. Primers C001 / C002 amplify the Cre gene, while primers C003 / C004 amplify an endogenous mouse gene, acting as an internal positive control

Primers

C001 : 5'-acc agc cag cta tca act cg-3'
C002 : 5'-tta cat tgg tcc agc cac c-3'
C003 : 5'-cta ggc cac aga att gaa aga tct-3'
C004 : 5'-gta ggt gga aat tct agc atc atc c-3'

Product Sizes

Primer Combination Product Size
C001 / C002 199 bp    Cre
C003 / C003 300 bp    Internal Control

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2 mM
2.5mM dNTP Mix 0.2mM
100 µMC001 0.5 µM
100 µMC002 0.5 µM
100 µMC003 0.5 µM
100 µMC004 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.