Strain Protocols

Strain Information
Strain Code: 01XG7
Strain Name: B6.129-Cdkn2atm1Cjs/Nci
Gene Symbol: Cdkn2a
Protocol Information
Allele: Cdkn2a<tm1Cjs>
Protocol Number: 1
Introductory Comment:

Primers

C018 : 5'-agt aca gca gcg gga gca tgg-3'
C019 : 5'-ttg agg agg acc gtg aag ccg-3'
C020 : 5'-acc aca ctg ctc gac att ggg-3'

Product Sizes

Primer Combination Product Size
C018 / C019 415 bp    Wildtype
C020 / C019 250bp    Knockout

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 1.5 mM
2.5mM dNTP Mix 0.2 mM
100 µMC018 0.5 µM
100 µMC019 0.5 µM
100 µMC020 0.5 µM
5U/┬Ál Taq Pol 0.015 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 1:00
65ºC 2:00 35 Cycles
72ºC 1:00
0ºC
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.