Strain Protocols

Strain Information
Strain Code: 01XG4
Strain Name: B6;D2-Tg(LPV-TAg121)2Tvd/Nci
Gene Symbol: TAg
Protocol Information
Allele: Tg(LPV-TAg121)2Tvd
Protocol Number: 1
Introductory Comment:

Primers

T026 : 5'-GAA TcT TTG cAG cTA ATG GAc c-3'
T027 : 5'-GcA Tcc cAG AAG cTc cAA AG-3'

Product Sizes

Primer Combination Product Size

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMT026 0.5 µM
100 µMT027 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.