Strain Protocols

Strain Information
Strain Code: 01XE4
Strain Name: FVB.129-Cdkn2atm2.1Rdp/Nci
Gene Symbol: Cdkn2a
Protocol Information
Allele: Cdkn2a<tm2.1Rdp>
Protocol Number: 1
Introductory Comment:

Primers

C015 : 5'-ggc aaa tag cgc cac cta t-3'
C016 : 5'-gac tcc atg ctg ctc cag at-3'
C017 : 5'-gcc gct gga cct aat aac ttc-3'

Product Sizes

Primer Combination Product Size
C015 / C016 189 bp    Wildtype
C017 / C016 243 bp    Knockout

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMC015 0.5 µM
100 µMC016 0.5 µM
100 µMC017 0.5 µM
5U/┬Ál Taq Pol 0.015 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 0:30
65ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.