Strain Protocols

Strain Information
Strain Code: 01XD4
Strain Name: C3H/He-Tg(RIP1-Tag5)5Dh/Nci
Gene Symbol: TAg
Protocol Information
Allele: Tg(RIP1-Tag5)5Dh
Protocol Number: 1
Introductory Comment:

Primers

T018 : 5'-GGA cAA Acc AcA AcT AGA ATG cAG-3'
T020 : 5'-cAc cGG AGA ATG GGA AGc cGA A-3'
T019 : 5'-cAG AGc AGA ATT GTG GAG TGG-3'
T021 : 5'-Tcc AcA cAG ATG GAG cGT ccA G-3'

Product Sizes

Primer Combination Product Size

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMT018 0.25 µM
100 µMT020 0.25 µM
100 µMT019 0.25 µM
100 µMT021 0.25 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.