Strain Protocols

Strain Information
Strain Code: 01XD3
Strain Name: B6.Cg-Tg(S100b-v-erbB)4496Waw Cdkn2atm1Rdp/Nci
Gene Symbol: v-erbB
Protocol Information
Allele: Tg(s100B-v-erbB)4496Waw
Protocol Number: 1
Introductory Comment:

Primers

E001 : 5'-cTc AcA GcA ATc TcA AAG cTc ccc-3'
E002 : 5'-AGc cTc cAA AGT cAG GTT GAT GAG c-3'

Product Sizes

Primer Combination Product Size
E001 / E002 525 bp    Trainsgene

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µME001 0.5 µM
100 µME002 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.