Strain Protocols

Strain Information
Strain Code: 01XC7
Strain Name: FVB;129-Men1tm1.1Ctre/Nci
Gene Symbol: Men1
Protocol Information
Allele: Men1<tm1.1Ctre>
Protocol Number: 1
Introductory Comment:

Primers

M014 : 5'-ccc AcA Tcc AGT ccc TcT TcA GcT-3'
M015 : 5'-ccc TcT GGc TAT TcA ATG GcA GGG-3'
M016 : 5'-cAT AAA ATc GcA GcA GGT GGG cAA-3'

Product Sizes

Primer Combination Product Size
M014 / M016 638 bp    Knockout
M014 / M015 300 bp    Wildtype

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMM014 0.33 µM
100 µMM015 0.33 µM
100 µMM016 0.33 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.