Strain Protocols

Strain Information
Strain Code: 01XBU
Strain Name: B6.129(Cg)-Cdkn2atm2.1Nesh/Nci
Gene Symbol: Cdkn2a
Protocol Information
Allele: B6.129(Cg)-Cdkn2a<tm2.1Nesh>/N
Protocol Number: 1
Introductory Comment:

Primers

Y384 : 5'-GTATGCTATACGAAGTTATTAGGTACTGC-3'
Y386 : 5'-CTATGTCAGATTTGGCTAGGGAGT-3'
Y385 : 5'-GTTTTGGAGCAGCAGGGATT-3'

Product Sizes

Primer Combination Product Size
Y384 / Y386 261bp    Lox
Y385 / Y386 194bp    Wild Type

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2
2.5mM dNTP Mix 0.2mM
100 µMY384 0.5 µM
100 µMY386 0.5 µM
100 µMY385 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.