Strain Protocols

Strain Information
Strain Code: 01XBL
Strain Name: B6;129-Myf6tm2(cre)Mrc/Nci
Gene Symbol: Cre
Protocol Information
Allele: Myf6<tm2(Cre)Mrc>
Protocol Number: 1
Introductory Comment:

Primers

ck219 : 5'-CTTCAGCGCCTTTCTTCCATCG-3'
ba88 : 5'-GAACTTCGTTTGCAACTGAC-3'
ba86 : 5'-GGATAGTGAAACAGGGCAA-3'

Product Sizes

Primer Combination Product Size
ck219 / ba88 WT    420 bp
ba86 / ba88 MT    290 bp

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2 mM
2.5mM dNTP Mix 0.2mM
100 µMck219 0.5 µM
100 µMba88 0.5 µM
100 µMba86 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
55ºC 0:30 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.