Strain Protocols

Strain Information
Strain Code: 01XB1
Strain Name: B6.129-Cdkn2atm1Rdp/Nci
Gene Symbol: Cdkn2a
Protocol Information
Allele: Cdkn2a<tm1Rdp>
Protocol Number: 1
Introductory Comment:

Primers

I001 : 5'-GTG ATc ccT cTA cTT TTT cTT cTG AcT T-3'
I002 : 5'-cGG AAc GcA AAT ATc GcA c-3'
I003 : 5'-GAG AcT AGT GAG AcG TGc TAc TTc cA-3'

Product Sizes

Primer Combination Product Size
I001 / I003 313 bp    Knockout
I001 / I002 278 bp    Wildtype

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMI001 0.33 µM
100 µMI002 0.33 µM
100 µMI003 0.33 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.