Strain Protocols

Strain Information
Strain Code: 01XA8
Strain Name: B6.Cg-Tg(Wap-Cre)11738Mam/Nci
Gene Symbol: Cre
Protocol Information
Allele: Tg(Wap-Cre)11738Mam
Protocol Number: 2
Introductory Comment: This strain contains a Cre recombinase gene linked to the WAP promoter. The genotying strategy is to detect this transgene with primer W003, which is specific for the WAP, and C031, which is specific for the Cre recombinase gene.

Primers

W003 : 5'-tag agc tgt gcc agc ctc ttc-3'
C031 : 5'-cat cac tcg ttg cat cga cc-3'
Diagram showing the primer relataionships for Strain 01XA8.  This strain contains a Cre recombinase gene linked to the WAP promoter.  The genotying strategy is to detect this transgene with primer W003, which is specific for the WAP, and C031, which is specific for the Cre recombinase gene.

Product Sizes

Primer Combination Product Size
W003 / C031 210 bp    WAP-Cre Tg

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMW003 0.5 µM
100 µMC031 0.5 µM
5U/┬Ál Taq Pol 0.015 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
0ºC
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 0:30
0ºC
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.