Strain Protocols

Strain Information
Strain Code: 01X61
Strain Name: B6D2F1-Tg(WAPT121)66Tvd/Nci
Gene Symbol: T121
Protocol Information
Allele: Tg(WAPT121)66Tvd
Protocol Number: 1
Introductory Comment: These mice express a mutant, truncated form of the SV40 TAg (T121) element under the control of the whey acidic protein (WAP) promoter. This protocol will not distinguish hemizygous mice from homozygous mice.

Primers

T012 : 5'-gaa tct ttg cag cta atg gac c-3'
T013 : 5'-gca tcc cag aag ctc caa ag--3'
Diagram showing the primer relataionships for Strain 01X61.  These mice express a mutant, truncated form of the SV40 TAg (T121) element under the control of the whey acidic protein (WAP) promoter.  This protocol will not distinguish hemizygous mice from homozygous mice.

Product Sizes

Primer Combination Product Size
T012 / T013 160 bp    Tg (T121)

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0mM
2.5mM dNTP Mix 0.2mM
100 µMT012 0.5 µM
100 µMT013 0.5 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 0:30
60ºC 0:30 35 Cycles
72ºC 0:30
72ºC 3:00
4ºC HOLD

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.