Strain Protocols

Strain Information
Strain Code: 01P05
Strain Name: C3;B6-Tg(CTSG-PML/RARa)135Ley/Nci
Gene Symbol: PML/RARA
Protocol Information
Allele: Tg(CTSG-PML/RARA)135Ley
Protocol Number: 1
Introductory Comment: This strain carries a chimeric promyelocytic leukemia-retinoic acid receptor alpha transgene (PML-RARa) under the of the human cathepsin G gene. The strategy of this genotyping protocol is to detect the human cathepsin G gene with primers P005 and P006. Primers P007 and P008 are included as an internal PCR control and amplify the endogenous mouse colony stimulating factor 3 receptor gene (csf3r).


P005 : 5'-GGc cTG Acc TcA Tcc cAT AG-3'
P007 : 5'-cTG cTc TTG AcA TAA Gcc TG-3'
P006 : 5'-Gcc cTT TTc ccc ATc cTA GG-3'
P008 : 5'-TAG cAG cAc TcT TcA ATG Gc-3'

Product Sizes

Primer Combination Product Size
P005 / P006 520 bp    CTSG Promoter
P007 / P008 360 bp    Internal Control

Master Mix

Reagent Final Concentration
10X Buffer 1X
25mM MgCl2 2.0 mM
2.5mM dNTP Mix 0.2 mM
100 µMP005 0.25 µM
100 µMP007 0.25 µM
100 µMP006 0.25 µM
100 µMP008 0.25 µM
5U/┬Ál Taq Pol 0.025 U/µl
Use 50 - 100 ng of DNA per reaction

Cycling Conditions

(Time in minutes:seconds)
Temperature Time Label
94ºC 3:00
94ºC 1:00
60ºC 2:00 35 Cycles
72ºC 1:00
72ºC 3:00

Disclaimer: PCR conditions were optimized using QIAGEN-purified mouse tail DNA and a PTC-200 thermocycler (MJ Research). Optimal conditions may vary with DNA quality or equipment used.