Standard Operating Procedures

To download a PDF of a standard operating procedure, click on the corresponding document number. The PDF will then open in a new window, and you can download it from there.

Equipment

 
Document Number Title
HSL_EQ_001 Biosafety Cabinet (BSC) Use and Maintenance
HSL_EQ_002 Operation, Use and Maintenance of CO2 Incubators
HSL_EQ_003 Use and Maintenance of the Thermo Fisher Sorvall Legend XTR Centrifuge in the HPV Serology Laboratory
HSL_EQ_004 Use and Maintenance of a BioTek Plate Washer in the HPV Serology Laboratory
HSL_EQ_005 Use and Maintenance of a Molecular Devices M5 Plate Reader in the HPV Serology Laboratory
HSL_EQ_006 Use and Maintenance of the Cellometer Auto 2000
HSL_EQ_007 Use and Maintenance of a 2-8C Refrigerator in the HPV Serology Laboratory
HSL_EQ_008 Use and Maintenance of the -80C Freezers in the HPV Serology Laboratory
HSL_EQ_009 Use and Maintenance of the Liquid Nitrogen Freezer
HSL_EQ_010 Use and Maintenance of the Fisher Scientific Isotemp GDP10 Water Bath
HSL_EQ_011 Use and Maintenance of the Forma Scientific Orbital Shaker
HSL_EQ_012 Use and Maintenance of Pipettes in the HPV Serology Laboratory
HSL_EQ_013 Use and Maintenance of the ThermoScientific Thermal Mixer
HSL_EQ_014 Use and Maintenance of a pH Meter
HSL_EQ_015 Use and Maintenance of an Analytical & Precision Balance
HSL_EQ_016 Use and Maintenance of -20C Freezers in the HPV Serology Laboratory
HSL_EQ_017 Use and Maintenance of a Laboratory Convection Oven
HSL_EQ_018 Use and Maintenance of an Inverted Microscope
HSL_EQ_019 Use and Maintenance of the Milli-Q Integral 3 Water System
HSL_EQ_020 Use and Maintenance of the Eppendorf Centrifuge
HSL_EQ_021 Use and Maintenance of NanoDrop 1000 Spectrophotometer
HSL_EQ_022 Controlled-Rate Cell Freezing Using a CoolCell Device
HSL_EQ_023 Use and Maintenance of a Compact Digital Microplate Shaker
HSL_EQ_024 Use and Maintenance of the Optima XPN Ultracentrifuge System
HSL_EQ_025 Use and Maintenance of the Combination Medical Unit
HSL_EQ_026 Use and Maintenance of the BioRad BioPlex 100
HSL_EQ_027 Use and Maintenance of the Agilent Bioanalyzer
HSL_EQ_028 Use and Maintenance of the Luminex FlexMap 3D
HSL_EQ_029 Use and Maintenance of the Innova 43 Incubator

Laboratory Assays

Document Number Title
HSL_LAB_001 293TT Cell Culturing and Maintenance
HSL_LAB_002 Preperation of Plasmid Bacterial Stock for Storage in Glycerol
HSL_LAB_003 Serum Sample Procedure
HSL_LAB_004 Plasmid Purification using a Qiagen Kit
HSL_LAB_005 Standard HPV pDNA Transfection in HEK293TT for VLP Production
HSL_LAB_006 HPV Neutralization Assay for Titer Determination
HSL_LAB_007 HPV Antibody ELISA
HSL_LAB_008 Bacterial Cell Culture Growth Measurement Using the GeneQuant Calculator
HSL_LAB_009 Use of the Pierce BCA Protein Assay Kit
HSL_LAB_010 Acrylamide Protein Gel Analysis of HPV Virus-Like Particles (VLPs)
HSL_LAB_011 Measurement of Purified Virus-Like Particles (VLP) Protein using the Bioanalyzer
HSL_LAB_013 Lipid-Mediated pDNA Ripcord Method of Transfection in HEK293TT for VLP Production
HSL_LAB_014 HPV VLP Conjugation to Luminex-based Microspheres
HSL_LAB_015 ELISA to Determine VLP Specificity
HSL_LAB_016 DNA Quantitation Using the NanoDrop

General Laboratory Procedures

Document Number Title
HSL_GL_006 Reagent Preperation for the HPV Serology Laboratory

Note: Procedures should only be used as guidance documents and not substituted for your own institute's/organization's/government's policies.

Please contact Troy Kemp (kemptj@mail.nih.gov, 301-846-6383) for any additional questions about the standard operating procedures.

Meeting Presentations

HPV Serology Standardization Update Meeting
The 32nd International Papillomavirus Conference (IPVC 2018) 
October 4th, 2018, Sydney, Australia

1.) HPV Serology standardization initiative update, Troy Kemp, Frederick National Laboratory
2.) Qualification and validation of multiplex assays for HPV Serology using the MSD platform, Gitika Panicker, CDC
3.) Development and use of primary International Standards, Joakim Dillner, Karolinska Institute

Please contact Troy Kemp (kemptj@mail.nih.gov, 301-846-6383) for the PowerPoint presentations from this meeting.