Services

• Focuses on retroviral virions and related samples. 
• Uses novel high-performance liquid chromatography methods for the separation of retroviral virions into their constituent proteins. 
• Supports National Institutes of Health and extramural researchers. 

• Non-commercially available fluorochrome-labeled antibodies developed by the Cellular Immunity Core. 
  • SIV Env Clone 7D3 antibody, labeled with Alexa Fluor 647 
  • SIV Gag Clone 55-2F12 antibody, labeled with FITC 

• Derive from infectious stock or infectious molecular clone (preferred)
• Typically infect SUPT1 based cell line
• Derive stable, productively infected cell line
• May derive limiting dilution clone
• Produce and analyze purified virus preparation(s) to determine virus yield and biochemical phenotype.
• Test for contamination by adventitious agents: bacteria, mold, or mycoplasma.

• 30 ml to 30 L batch sizes
• Purify by sucrose density gradient ultracentrifugation
• Provide in infectious (non-treated) or chemically inactivated (using Aldrithiol-2)

Provides cryo-EM images from submitted specimens for cancer research. 

Submit an application

Researchers can request the following services from the Imaging Mass Cytometry Laboratory:  

• Building the metal-label antibody panel targeted to the project goals  
• Metal-labeling and validation of in-house conjugated and commercially available metal-label antibodies with known positive and negative control tissue slides.  
• Staining and acquisition of required number of tissue slides/tissue microarrays.  
• Sharing raw data from the instrument and assistance with high dimensional data analysis. 

If you are interested in requesting a service from the Imaging Mass Cytometry Laboratory, please contact Milind Pore or send an email to IMCFNLCR@nih.gov.

The Nanotechnology Characterization Laboratory Assay Cascade is a free characterization program available to any researcher creating a nano-product for cancer. Accepted applicants will have their nanomedicine formulation characterized for physicochemical traits, and immunology, pharmacology and toxicology properties.  

Who can request the service

The program is open to developers focused on oncology from academia, industry, and government. Applicants from non-U.S. organizations are welcome to apply. This service is funded by the National Cancer Institute. 

Application process  

Applications are accepted year-round with quarterly deadlines the first business days of March, June, September and December. 

Submit Application

Various research and development projects can be supported, including:  

• Formulation, Optimization, Lead Selection 
• Mechanistic Studies 
• Characterization of Non-Oncology Nanomaterials 
• Bioanalytical Assay Development 
• Instrument Optimization 
• And more 

Who can request the service 

These projects are open to all nanotech developers. All research and development projects must be fully funded by the submitting investigator.  

How to apply

Contact us for more information or to discuss a potential project. 

Work is initiated through negotiated Contractor Cooperative Research and Development Agreements (cCRADAs), which are organized through our Partnership Development Office. 

• Imaging software  
• Machine learning, deep learning and AI 
• Bioinformatics workflows 
• Sequence annotation algorithms 
• Cryo EM pipeline 
• Power analysis 

Code Repositories

• Biomedical annotation and visualization resources 
• Genomic workflows 

Scientific Applications