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Services
We offer access to specialized technologies and select science services. Laboratories providing services to external researchers leverage their expertise and facilities to work with you.
Technical services are managed through the Partnership Development Office and are available through specific contracts.
HIV/SIV Services
- HIV/SIV Single Genome Amplification and Sequencing Technical Service FNL Technical Service Number: ACVP-05
- Next-generation sequencing analysis of barcoded SIV, SHIV, or HIV Technical Service FNL Technical Service Number: ACVP-15
- Novel CD4+ T Cell Specific Immunohistochemistry Detection and Analysis Technical Service FNL Technical Service Number: ACVP-03
- Quantitative Assessment of Lymphoid Tissue Fibrosis Technical Service FNL Technical Service Number: ACVP-11
- Quantitative assessment of HIV or SIV viral DNA in laser capture microdissected immune cells Technical Service FNL Technical Service Number: ACVP-12
- Lineage-specific Next Gen Multiplex In Situ Hybridization for HIV or SIV RNA and DNA Technical Service FNL Technical Service Number: ACVP-14
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Retroviral protein purification and characterization
Description:
- Focuses on retroviral virions and related samples.
- Uses novel high-performance liquid chromatography methods for the separation of retroviral virions into their constituent proteins.
- Supports National Institutes of Health and extramural researchers.
- Plasma or CSF SIV/SHIV RNA measurements Technical Service FNL Technical Service Number: ACVP-02, ACVP-13
- Cellular or Tissue SIV/SHIV RNA and DNA testing Technical Service FNL Technical Service Number: ACVP-06
- Detection of Mycobacterium tuberculosis DNA Technical Service FNL Technical Service Number: ACVP-16
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Novel nonhuman primate and SIV flow cytometry reagents
Description:
- Non-commercially available fluorochrome-labeled antibodies developed by the Cellular Immunity Core.
- SIV Env Clone 7D3 antibody, labeled with Alexa Fluor 647
- SIV Gag Clone 55-2F12 antibody, labeled with FITC
- Non-commercially available fluorochrome-labeled antibodies developed by the Cellular Immunity Core.
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Production and purification of HIV, SIV, or control microvesicles
Description:
- 30 ml to 30 L batch sizes
- Purify by sucrose density gradient ultracentrifugation
- Provide in infectious (non-treated) or chemically inactivated (using Aldrithiol-2)
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Development of productively infected cell lines or cell clones
Description:
- Derive from infectious stock or infectious molecular clone (preferred)
- Typically infect SUPT1 based cell line
- Derive stable, productively infected cell line
- May derive limiting dilution clone
- Produce and analyze purified virus preparation(s) to determine virus yield and biochemical phenotype.
- Test for contamination by adventitious agents: bacteria, mold, or mycoplasma.
Imaging
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Cryo-electron microscopy
Description:
Provides cryo-EM images from submitted specimens for cancer research.
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Imaging mass cytometry
Description:
Researchers can request the following services from the Imaging Mass Cytometry Laboratory:
- Building the metal-label antibody panel targeted to the project goals
- Metal-labeling and validation of in-house conjugated and commercially available metal-label antibodies with known positive and negative control tissue slides.
- Staining and acquisition of required number of tissue slides/tissue microarrays.
- Sharing raw data from the instrument and assistance with high dimensional data analysis.
If you are interested in requesting a service from the Imaging Mass Cytometry Laboratory, please contact Milind Pore or send an email to IMCFNLCR@nih.gov.
Nanomedicines and Nanoformulation
- Pharmacokinetic study of nanomedicine in rats Technical Service FNL Technical Service Number: NCL-02
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Assay Cascade: Preclinical nanotechnology characterization
Description:
The Nanotechnology Characterization Laboratory Assay Cascade is a free characterization program available to any researcher creating a nano-product for cancer. Accepted applicants will have their nanomedicine formulation characterized for physicochemical traits, and immunology, pharmacology and toxicology properties.
Who can request the service
The program is open to developers focused on oncology from academia, industry, and government. Applicants from non-U.S. organizations are welcome to apply. This service is funded by the National Cancer Institute.
Application process
Applications are accepted year-round with quarterly deadlines the first business days of March, June, September and December.
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Nanotechnology research and development projects
Description:
Various research and development projects can be supported, including:
- Formulation, Optimization, Lead Selection
- Mechanistic Studies
- Characterization of Non-Oncology Nanomaterials
- Bioanalytical Assay Development
- Instrument Optimization
- And more
Who can request the service
These projects are open to all nanotech developers. All research and development projects must be fully funded by the submitting investigator.
How to apply
Contact us for more information or to discuss a potential project.
Work is initiated through negotiated Contractor Cooperative Research and Development Agreements (cCRADAs), which are organized through our Partnership Development Office.
- An in vitro drug release study in human plasma Technical Service FNL Technical Service Number: NCL-01
Laboratory Animal Services
- Use of Mouse Embryonic Stem Cells Expressing miRNAs Technical Service FNL Technical Service Number: LASP-01
- In Vitro Cell Cytotoxicity Evaluation Technical Service FNL Technical Service Number: LASP-02, LASP-08
- Pharmacokinetic Evaluation in Kras/p53 PDAC Mice Technical Service FNL Technical Service Number: LASP-03
- Tolerance Evaluation of Experimental Compound in Kras/p53 PDAC Mice Technical Service FNL Technical Service Number: LASP-04
- Time Course Assessment of Tumor Response to Therapeutics Technical Service FNL Technical Service Number: LASP-05, LASP-09
- Assessment of Drug Efficacy in a Survival Study Technical Service FNL Technical Service Number: LASP-06, LASP-07, LASP-10, LASP-11
Other
- mRNA Isolation and Expression Analysis in Human B-cell Lymphoma Samples Technical Service FNL Technical Service Number: CRTP-13
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GitHub repository for developed software
Description:
- Imaging software
- Machine learning, deep learning and AI
- Bioinformatics workflows
- Sequence annotation algorithms
- Cryo EM pipeline
- Power analysis
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Biomedical applications developed by the Advanced Biomedical Computational Science groups
Description:
- Biomedical annotation and visualization resources
- Genomic workflows
Intramural Investigators
Investigators from the National Institutes of Health can access available services through the National Cancer Institute at Frederick Accessioning System (NAS) or the National Institutes of Health Collaborative Research Exchange (CREX). Both are methods for requesting certain services from the Frederick National Laboratory by the National Institutes of Health Intramural Research Program.