Available Strain Details

Strain Information
Common Strain Name: K14-HPV16 Pricing and Ordering Information
Strain Nomenclature: FVB.Cg-Tg(KRT14-HPV16)wt1Dh/Nci
Animal State: Frozen Embryo & Frozen Sperm
Release Category (Required for MTA form): B1

Strain Description: A 2 kb fragment of the human keratin 14 (KRT14) promoter/enhancer was used to drive expression of human papillomavirus type 16 (HPV16) oncoproteins in basal squamous epithelial cells. Tumorigenesis in this model was hormone-dependent. Chronic estradiol treatment at 0.1-0.25 mg/60-day induced invasive squamous cancers predominantly in the vulva, vagina and outer cervix of K14-HPV16 mice. None of the tumors were metastatic. At low estradiol dose (0.05 mg/60-day) squamous cancers were almost exclusively localized to the transformation zone situated between the upper cervix and lower uterus. Metaplastic foci appeared to arise from single cells located at the epithelial-stromal interface, potentially glandular reserve cells. Epithelial dysplasia and metaplasia were observed after 4 months of estradiol treatment, leading to high-grade dysplasia and multifocal carcinomas by 6 months. Spontaneous skin cancer was observed in 40% of mice between 8-12 months of age on chest or ear.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: human papillomavirus type 16
Transgene Symbol: HPV16
Promotor Name: keratin 14 (KRT14), human
Promotor Symbol: KRT14
Current Genetic Background: FVB.Cg
Approx. Generation: 34
Organ Site: Skin, Cervix
Additional Information
Cryopreservation Mating Scheme: EMBRYOS: The cryopreservation mating scheme is FVB females x hemizygous males. SPERM: Cryopreserved sperm was obtained from hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB (male or female).
Genotyping Information: Protocol 1: Allele: Tg(KRT14-HPV16)wt1Dh
Donating Investigator: Jeffrey Arbeit

Key Reference: Arbeit JM, Munger K, Howley PM, Hanahan D. Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice. J Virol 68, 4358-4368, 1994. PMID: 7515971 [Pubmed Abstract]

Related Reference: Elson DA, Riley RR, Lacey A, Thordarson G, Talamantes FJ, Arbeit JM. Sensitivity of the cervical transformation zone to estrogen-induced squamous carcinogenesis. Cancer Res 60, 1267-1275, 2000. PMID: 10728686 [Pubmed Abstract]