Available Strain Details

Strain Information
Common Strain Name: Apc Delta 580 Pricing and Ordering Information
Strain Nomenclature: STOCK-Apctm2.1Rak/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: This strain carries a germline targeted mutant allele of Apc in which exon14 has been removed by cre-mediated recombination, leading to expression of a truncated Apc protein of 605 amino acid residues (aa), of which the first 580 aa correspond to the normal protein. Heterozygous mice develop multiple intestinal neoplasias and die within 5 months of age. Homozygotes are embryonic lethal. Heterozygous females show nursing deficiency.
Mutation Information
Mutation Type: Targeted
Gene Name: Adenomatosis polyposis coli
Gene Symbol: Apc
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: STOCK
Approx. Generation: 10
Organ Site: GI tract
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote male x wildtype C57BL/6 female. Average litter size is 7. Homozygotes are embryonic lethal. Heterozygous females don't nurse well.
Genotyping Information: Protocol 1: Allele: Apc<tm2.1Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Kuraguchi M, Wang XP, Bronson RT, Rothenberg R, Ohene-Baah NY, Lund JJ, Kucherlapati M, Maas RL, Kucherlapati R. Adenomatous polyposis coli (Apc) is required for normal development of skin and thymus. PLoS Genetics 2, 1362-1374, 2006. PMID 17002498 [Pubmed Abstract]