Available Strain Details

Strain Information
Common Strain Name: K5/tTA Pricing and Ordering Information
Strain Nomenclature: FVB/N-Tg(KRT5-tTA)1216Glk/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B1

Strain Description: The doxycycline-dependent transcriptional repressor, tTA, was placed under control of the bovine keratin 5 (K5, KRT5) promoter. When tested on a tetO-LacZ transgenic background, doxycycline suppressed expression of beta-galactosidase in a tissue-specific manner. Reduced beta-galactosidase activity was detected in skin (basal layer of the epidermis and hair follicles), tongue, forestomach, and trachea. No reduction was seen in tissues that do not express endogenous keratin 5 (liver, spleen, muscle, heart, intestine).
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: doxycylin-dependent transcriptional repressor, tTA
Transgene Symbol: KRT5
Promotor Name: keratin 5 (bovine)
Promotor Symbol: K5
Current Genetic Background: FVB/N
Approx. Generation: 10
Organ Site: Multiple
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB mate (male or female). Average litter size is 6-9.
Genotyping Information: Protocol 1: Allele: Tg(KRT5-tTA)1216Glk
Donating Investigator: Adam Glick

Key Reference: Diamond I, Owolabi T, Marco M, Lam C, Glick A. Conditional gene expression in the epidermis of transgenic mice using the tetracyclin-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol 115, 788-794, 2000. PMID: 11069615 [Pubmed Abstract]

Related Reference: Liu X, Alexander V, Vijayachandra K, Bhogte E, Diamond I, Glick A. Conditional epidermal expression of TGFbeta1 blocks neonatal lethality but causes a reversible hyperplasia and alopecia. Proc Natl Acad Sci USA 98, 9139-9144, 2001. PMID: 11481479 [Pubmed Abstract]

Sponsor: National Cancer Institute