Available Strain Details

Strain Information
Common Strain Name: ArfGFP Pricing and Ordering Information
Strain Nomenclature: B6.129S1-Cdkn2atm1(GFP)Cjs/Nci
Animal State: Frozen Embryo
Release Category (Required for MTA form): B3

Strain Description: A GFP reporter cDNA was inserted by homologous recombination into the Arf-specific exon 1 beta of the Cdkn2a locus. Expression of p19ARF is disrupted and GFP is expressed under control of the endogenous Arf promoter. Homozygous Arf animals spontaneously developed tumors by 16 weeks of age. The predominant tumor types were highly invasive sarcomas of various types (65%) and lymphomas (33%), similar to Arf-null mice (see NCI Mouse Repository strains 01XF6 and 01XG7). Depending on tumor type, size, and anatomical location, many solid tumors exhibited vivid green fluorescence, and metastatic lymphomas sometimes appeared macroscopically as green fluorescent nodules in the liver, indicating that the Arf promoter remained active in late stages of tumor development. The ArfGFP mutation allows for tumor progression to be monitored by whole-body fluorescence imaging.
Mutation Information
Mutation Type: Targeted
Gene Name: Cyclin-dependent kinase inhibitor 2A
Gene Symbol: Cdkn2a
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129S1
Approx. Generation: 9
Organ Site: Multiple
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is 6-8.
Genotyping Information: Protocol 1: Allele: Cdkn2a<tm1(GFP)Cjs>
Donating Investigator: Dr. Charles Sherr

Key Reference: Zindy F, Williams RT, Baudino TA, Rehg JE, Skapek SX, Cleveland JL, Roussel MF, Sherr CJ. 2003. Arf tumor suppressor promoter monitors latent oncogenic signals in vivo. Proc Natl Acad Sci USA 100, 2003, 15930-15935. PMID: 14665695 [Pubmed Abstract]